IDENTITY Ostreococcus_tauri Domain: EukaryotaDivision: ChlorophytaClass: MamiellophyceaeOrder: MamiellalesFamily: BathycoccaceaeGenus: Ostreococcus Clade: clade CStrain name: (OTTH 0595) LHY Authentic culture: STATUS Clonal: Axenic: Pure: Heterotrophic: Toxic: Symbiotic: Transformed: Substitute strain (e.g. for cryopreserved or lost strains): RCC745 MORPHOLOGYCell shape: coccoidLength: 1.00Cell motility: non-motile CULTURE CONDITIONSRCC medium: KRCC temperature: 20.00RCC transfer period weeks: 2.00RCC light: 20Remark: Transgenic lines made in the OTTH0595 strain( (RCC745) with the pOtLuc expression vector with expression of the Luciferase protein under the control of the circadian clock promoter LHY. Corellou F, Schwartz C, Motta JP, el Djouani-Tahri B, Sanchez F. , et al (2009) Clocks in the green lineage: comparative functional analysis of the circadian architecture of the picoeukaryote Ostreococcus. Plant Cell 21: 3436-3449. doi:10.1105/tpc.109.068825. Transformation Protocol: Gerben van Ooijen, Kirsten Knox, Katalin Kis, Francois-Yves Bouget, Andrew J. Millar (2012) Genomic transformation of the picoeukaryote Ostreococcus tauri. J. Vis. Exp. 65: e4074. doi:10.3791/4074. RCC Staff >Details for RCC staff onlyDate entered catalog: Saturday, December 3, 2016Mother strain: RCC745Deposit to RCC date: Wednesday, November 30, 2016Deposit to rcc by: Sarah Hodge - University of EdinburghTransformed by: Sarah Hodge - University of EdinburghTransformed methods: Transgenic lines made in the OTTH0595 strain with the pOtLuc expression vector with expression of the Luciferase protein under the control of the circadian clock promoter LHY. Corellou F, Schwartz C, Motta JP, el Djouani-Tahri B, Sanchez F. , et al (2009) Clocks in the green lineage: comparative functional analysis of the circadian architecture of the picoeukaryote Ostreococcus. Plant Cell 21: 3436-3449. doi:10.1105/tpc.109.068825. Transforamtion Protocol: Gerben van Ooijen, Kirsten Knox, Katalin Kis, Fran?ois-Yves Bouget, Andrew J. Millar (2012) Genomic transformation of the picoeukaryote Ostreococcus tauri. J. Vis. Exp. 65: e4074. doi:10.3791/4074.
Ostreococcus_tauri Domain: EukaryotaDivision: ChlorophytaClass: MamiellophyceaeOrder: MamiellalesFamily: BathycoccaceaeGenus: Ostreococcus