List of the most common culture media used in the Roscoff Culture Collection (with links to the other web sites).
- Components for some media can be ordered from :
- If you do not have access to fresh seawater, Red Sea Salt (broken link) can be used to reconstitute sea water and seems to provide good results with marine cyanobacteria Synechococcus. Alternatively you may order sea water from NCMA (if you can afford it...).
For all media
- Leave the seawater (SW) to age for 2 months at room temperature;
Note : This"aging" step is critical (but akin to black magic) because during that period bacteria present in the sea water will degrade many organic molecules that are probably detrimental to algae. Our experince shows that medium made with 'fresh' seawater will in many cases prevent cells from growing.
- Filter SW through 0.2µm membrane filter and autoclave;
- Autoclave the different nutrient solutions;
- Asceptically add the nutrients to the SW and store at 20°C.