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Culture Media

List of the most common culture media used in the Roscoff Culture Collection (with links to the other web sites).

  • Components for some media can be ordered from :
  • If you do not have access to fresh seawater, Red Sea Salt (broken link) can be used to reconstitute sea water and seems to provide good results with marine cyanobacteria Synechococcus. Alternatively you may order sea water from NCMA (if you can afford it...).

For all media

  • Leave the seawater (SW) to age for 2 months at room temperature;
    Note : This"aging" step is critical (but akin to black magic) because during that period bacteria present in the sea water will degrade many organic molecules that are probably detrimental to algae.  Our experince shows that medium made with 'fresh' seawater will in many cases prevent cells from growing.
  • Filter SW through 0.2µm membrane filter and autoclave;
  • Autoclave the different nutrient solutions;
  • Asceptically add the nutrients to the SW and store at 20°C.
Medium name Used for Medium file Remark Medium color
BG11 Freshwater cyanobacteria PDF icon Download pdf (110.87 KB) Appelé PCC-539 Rouge
Bold SW Some eukaryotes PDF icon Download pdf (33.78 KB) Made in sea water
f/2 Some photosynthetic eukaryotes (dinoflagellates,...) PDF icon Download pdf (111.07 KB) Orange
f/2 + Soil PDF icon Download pdf (104.37 KB)
JM SW Cryptophyceae PDF icon Download pdf (35.84 KB) Made in sea water
K Most photosynthetic eukaryotes PDF icon Download pdf (122.8 KB)
K+Si Diatoms PDF icon Download pdf (124.2 KB) Vert
K/2 PDF icon Download pdf (109.33 KB) K medium diluted twice Bleu
L1 Eukaryotes PDF icon Download pdf (593.78 KB) Receipe obtained from NCMA web site. This medium is a variation of K and f/2 and works very well for many species.
PCC-559 Prochlorococcus and other cyanobacteria PDF icon Download pdf (138.89 KB) From Pasteur Collection. We do not use it.
PCR-S11-Red Sea Prochlorococcus and Synechococcus cyanobacteria PDF icon Download pdf (147.32 KB) From Pasteur Collection Rose
Riz Heterotrophic eukaryotes PDF icon Download pdf (109.48 KB) Gris
SN Some Synechococcus cyanobacteria PDF icon Download pdf (119.57 KB)
YEP Schizochytrium PDF icon Download pdf (107.47 KB)