IDENTITY Ostreococcus_tauri Domain: EukaryotaDivision: ChlorophytaClass: MamiellophyceaeOrder: MamiellalesFamily: BathycoccaceaeGenus: Ostreococcus Clade: clade CStrain name: (OTTH 0595) LHY CK1 #4 Authentic culture: STATUS Clonal: Axenic: Pure: Heterotrophic: Toxic: Symbiotic: Transformed: Substitute strain (e.g. for cryopreserved or lost strains): RCC745 MORPHOLOGYCell shape: coccoidLength: 1.00Cell motility: non-motile CULTURE CONDITIONSRCC medium: KRCC temperature: 20.00RCC transfer period weeks: 2.00RCC light: 20Remark: Transgenic lines made in the (OTTH 0595) LHY strain using pOtox expression vector with O.t CK1 gene (Ostta02g03780) under the control of the constitutive HAPT promoter. van Ooijen G, Hindle M, Martin SF, Barrios-Llerena M, Sanchez F, Bouget F-Y, ONeill JS, Le Bihan T, Millar AJ (2013) Functional analysis of Casein Kinase 1 in a minimal circadian system. PLoS One, 8: e70021. Transformation Protocol: Gerben van Ooijen, Kirsten Knox, Katalin Kis, Francois-Yves Bouget, Andrew J. Millar (2012) Genomic transformation of the picoeukaryote Ostreococcus tauri. J. Vis. Exp. 65: e4074. doi:10.3791/4074. RCC Staff >Details for RCC staff onlyDate entered catalog: Saturday, December 3, 2016Mother strain: RCC745Deposit to RCC date: Wednesday, November 30, 2016Deposit to rcc by: Sarah Hodge - University of EdinburghTransformed by: Sarah Hodge - University of EdinburghTransformed methods: Transgenic lines made in the (OTTH 0595) LHY strain using pOtox expression vector with O.t CK1 gene under the control of the constitutive HAPT promoter. van Ooijen G, Hindle M, Martin SF, Barrios-Llerena M, Sanchez F, Bouget F-Y, O'Neill JS, Le Bihan T, Millar AJ (2013) Functional analysis of Casein Kinase 1 in a minimal circadian system. PLoS One, 8: e70021 Transforamtion Protocol: Gerben van Ooijen, Kirsten Knox, Katalin Kis, Fran?ois-Yves Bouget, Andrew J. Millar (2012) Genomic transformation of the picoeukaryote Ostreococcus tauri. J. Vis. Exp. 65: e4074. doi:10.3791/4074.
Ostreococcus_tauri Domain: EukaryotaDivision: ChlorophytaClass: MamiellophyceaeOrder: MamiellalesFamily: BathycoccaceaeGenus: Ostreococcus