Title | Sustainable Cultivation of Porphyridium cruentum via Agro-Industrial By-Products: A Study on Biomass and Lipid Enhancement |
Publication Type | Journal Article |
Year of Publication | 2024 |
Authors | Luca RGiovanni, Langellotti ALuca, Verardo V, Garcia BMartin, Oliviero M, Masi P |
Journal | Biocatalysis and Agricultural Biotechnology |
Pagination | 103341 |
Date Published | aug |
ISSN | 18788181 |
Keywords | RCC653 |
Abstract | This study explored the cultivation of Porphyridium cruentum using beet molasses and corn steep liquor (CSL) as alternative agri-waste substrates. The objective was to enhance the biomass and lipid production of P. cruentum, known for its potential as an industrial EPA producer. Using a BoxBehnken Design (BBD) as part of the response surface methodology (RSM), we investigated the effects of beet molasses, CSL, and F/2 nutrients on the microalga's cultivation. The results 24 demonstrated a significant influence of these factors on the final cell count and lipid content over an 25 8-day cultivation period. Specifically, optimal growth conditions were identified at approximately 26 1.78 g/L of molasses and 1.89 g/L of CSL, yielding a cell count of 12.1 x 106 cell/mL and a lipid 27 content of 24.48%. Validation experiments reaffirmed these findings, with observed results closely 28 aligning with predicted values. This research underscores the potential of using agro-industrial by29 products for large-scale cultivation of P. cruentum, offering a sustainable approach for enhancing 30 lipid production and other biotechnological applications. |
URL | https://linkinghub.elsevier.com/retrieve/pii/S1878818124003256 |
DOI | 10.1016/j.bcab.2024.103341 |