IDENTITY Ostreococcus_tauri Domain: EukaryotaDivision: ChlorophytaClass: MamiellophyceaeOrder: MamiellalesFamily: BathycoccaceaeGenus: Ostreococcus Clade: clade CStrain name: (OTTH 0595) TOC1-LUC #84 Authentic culture: STATUS Clonal: Axenic: Pure: Heterotrophic: Toxic: Symbiotic: Transformed: Substitute strain (e.g. for cryopreserved or lost strains): RCC745 MORPHOLOGYCell shape: coccoidLength: 1.00Cell motility: non-motile CULTURE CONDITIONSRCC medium: KRCC temperature: 20.00RCC transfer period weeks: 2.00RCC light: 20Remark: Transgenic lines made in the OTTH0595 strain with pOtox expression vector with TOC1 - Luciferase - 3FLAG 10His gene fusion under the control of constitutive promoter (HAPT1) TOC1 gene (Ostta13g01820 (unpublished). Information about vectors : Corellou F, Schwartz C, Motta JP, el Djouani-Tahri B, Sanchez F. , et al (2009) Clocks in the green lineage: comparative functional analysis of the circadian architecture of the picoeukaryote Ostreococcus. Plant Cell 21: 3436-3449. doi:10.1105/tpc.109.068825. Transformation Protocol: Gerben van Ooijen, Kirsten Knox, Katalin Kis, Francois-Yves Bouget, Andrew J. Millar (2012) Genomic transformation of the picoeukaryote Ostreococcus tauri. J. Vis. Exp. 65: e4074. doi:10.3791/4074. RCC Staff >Details for RCC staff onlyDate entered catalog: Saturday, December 3, 2016Mother strain: RCC745Deposit to RCC date: Wednesday, November 30, 2016Deposit to rcc by: Sarah Hodge - University of EdinburghTransformed by: Sarah Hodge - University of EdinburghTransformed methods: Transgenic lines made in the OTTH0595 strain with pOtox expression vector with TOC1 - Luciferase - 3FLAG 10His gene fusion under the control of constitutive promoter (HAPT1) (unpublished) Information about vectors Corellou F, Schwartz C, Motta JP, el Djouani-Tahri B, Sanchez F. , et al (2009) Clocks in the green lineage: comparative functional analysis of the circadian architecture of the picoeukaryote Ostreococcus. Plant Cell 21: 3436-3449. doi:10.1105/tpc.109.068825. Transforamtion Protocol: Gerben van Ooijen, Kirsten Knox, Katalin Kis, Fran?ois-Yves Bouget, Andrew J. Millar (2012) Genomic transformation of the picoeukaryote Ostreococcus tauri. J. Vis. Exp. 65: e4074. doi:10.3791/4074.
Ostreococcus_tauri Domain: EukaryotaDivision: ChlorophytaClass: MamiellophyceaeOrder: MamiellalesFamily: BathycoccaceaeGenus: Ostreococcus