@article {dedman_alterations_2024,
	title = {Alterations in metalloprotein abundance under ocean warming in the marine green alga Micromonas pusilla using the mebipred predictive tool},
	journal = {Algal Research},
	volume = {78},
	year = {2024},
	month = {mar},
	pages = {103412},
	abstract = {Metals are essential to life, required for the functioning of a substantial fraction of proteins. Marine phytoplankton drives the oceanic carbon cycle, influencing global biogeochemistry. These organisms rely on trace metal nutrients; however how trace nutrient demand will change under ocean warming is uncertain. The currently limited annotation of metalloproteins contributes to this lack of understanding. Herein, we utilise the recently published mebipred tool to predict the metalloproteome of the globally occurring green alga, Micromonas pusilla, revealing \~{}20~\% of its reference proteome to display ion-binding properties. Using the predicted metalloproteome, and existing Gene Ontology annotation, we present a comprehensive insight towards changes in metalloprotein abundance after exposure to ocean warming conditions (+6~{\textdegree}C), using a shotgun proteomic approach. We outline that predictions must be combined with existing annotation to fully capture the metalloprotein response. Approximately 50~\% of identified metalloproteins significantly altered in abundance under warming, largely related to changes in cellular function. Notably, warming appeared to shift Fe use from photosynthesis to storage in M. pusilla, indicated by a 2.36 log2 fold-increase in ferritin and decline in abundance of photosynthetic proteins. Ocean warming will inevitably alter cellular use and demand for metal cofactors in marine phytoplankton with possible implications for biogeochemical cycling. Bioinformatics tools such as mebipred greatly expand our ability to examine such changes.},
	keywords = {Biogeochemistry, marine phytoplankton, Ocean warming, proteomics, RCC1614, Trace metals},
	issn = {2211-9264},
	doi = {10.1016/j.algal.2024.103412},
	url = {https://www.sciencedirect.com/science/article/pii/S2211926424000249},
	author = {Dedman, Craig J. and Fournier, Marjorie and Rickaby, Rosalind E. M.}
}
@article {harcourt_intraspecific_2024,
	title = {Intraspecific trait variation modulates the temperature effect on elemental quotas and stoichiometry in marine Synechococcus},
	journal = {PLOS ONE},
	volume = {19},
	number = {3},
	year = {2024},
	note = {Publisher: Public Library of Science},
	month = {mar},
	pages = {e0292337},
	abstract = {Diverse phytoplankton modulate the coupling between the ocean carbon and nutrient cycles through life-history traits such as cell size, elemental quotas, and ratios. Biodiversity is mostly considered at broad functional levels, but major phytoplankton lineages are themselves highly diverse. As an example, Synechococcus is found in nearly all ocean regions, and we demonstrate contains extensive intraspecific variation. Here, we grew four closely related Synechococcus isolates in serially transferred cultures across a range of temperatures (16{\textendash}25{\textdegree}C) to quantify for the relative role of intraspecific trait variation vs. environmental change. We report differences in cell size (p<0.01) as a function of strain and clade (p<0.01). The carbon (QC), nitrogen (QN), and phosphorus (QP) cell quotas all increased with cell size. Furthermore, cell size has an inverse relationship to growth rate. Within our experimental design, temperature alone had a weak physiological effect on cell quota and elemental ratios. Instead, we find systemic intraspecific variance of C:N:P, with cell size and N:P having an inverse relationship. Our results suggest a key role for intraspecific life history traits in determining elemental quotas and stoichiometry. Thus, the extensive biodiversity harbored within many lineages may modulate the impact of environmental change on ocean biogeochemical cycles.},
	keywords = {Biogeochemistry, Cell enumeration techniques, Oceans, Particulates, Phylogenetics, phytoplankton, RCC1086, RCC2673, RCC515, Stoichiometry, Synechococcus},
	issn = {1932-6203},
	doi = {10.1371/journal.pone.0292337},
	url = {https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0292337},
	author = {Harcourt, Renne and Garcia, Nathan S. and Martiny, Adam C.}
}
@article {bordiga_unexpected_2023,
	title = {Unexpected silicon localization in calcium carbonate exoskeleton of cultured and fossil coccolithophores},
	journal = {Scientific Reports},
	volume = {13},
	number = {1},
	year = {2023},
	note = {Number: 1 Publisher: Nature Publishing Group},
	month = {may},
	pages = {7417},
	abstract = {Coccolithophores, marine calcifying phytoplankton, are important primary producers impacting the global carbon cycle at different timescales. Their biomineral structures, the calcite containing coccoliths, are among the most elaborate hard parts of any organism. Understanding the morphogenesis of coccoliths is not only relevant in the context of coccolithophore eco-physiology but will also inform biomineralization and crystal design research more generally. The recent discovery of a silicon (Si) requirement for crystal shaping in some coccolithophores has opened up a new avenue of biomineralization research. In order to develop a mechanistic understanding of the role of Si, the presence and localization of this chemical element in coccoliths needs to be known. Here, we document for the first time the uneven Si distribution in Helicosphaera carteri coccoliths through three synchrotron-based techniques employing X-ray Fluorescence and Infrared Spectromicroscopy. The enrichment of Si in specific areas of the coccoliths point to a targeted role of this element in the coccolith formation. Our findings mark a key step in biomineralization research because it opens the door for a detailed mechanistic understanding of the role Si plays in shaping coccolith crystals.},
	keywords = {Biogeochemistry, Marine biology, Palaeontology, RCC1323},
	issn = {2045-2322},
	doi = {10.1038/s41598-023-34003-3},
	url = {https://www.nature.com/articles/s41598-023-34003-3},
	author = {Bordiga, M. and Lupi, C. and Langer, G. and Gianoncelli, A. and Birarda, G. and Pollastri, S. and Bonanni, V. and Bedolla, D. E. and Vaccari, L. and Gariani, G. and Cerino, F. and Cabrini, M. and Beran, A. and Zuccotti, M. and Fiorentino, G. and Zanoni, M. and Garagna, S. and Cobianchi, M. and Di Giulio, A.}
}
@article {liu_phycosphere_2022,
	title = {Phycosphere pH of unicellular nano- and micro- phytoplankton cells and consequences for iron speciation},
	journal = {The ISME Journal},
	volume = {16},
	number = {10},
	year = {2022},
	note = {Number: 10 Publisher: Nature Publishing Group},
	month = {oct},
	pages = {2329{\textendash}2336},
	abstract = {Surface ocean pH is declining due to anthropogenic atmospheric CO2 uptake with a global decline of \textasciitilde0.3 possible by 2100. Extracellular pH influences a range of biological processes, including nutrient uptake, calcification and silicification. However, there are poor constraints on how pH levels in the extracellular microenvironment surrounding phytoplankton cells (the phycosphere) differ from bulk seawater. This adds uncertainty to biological impacts of environmental change. Furthermore, previous modelling work suggests that phycosphere pH of small cells is close to bulk seawater, and this has not been experimentally verified. Here we observe under 140 μmol photons{\textperiodcentered}m-2{\textperiodcentered}s-1 the phycosphere pH of Chlamydomonas concordia (5 {\textmu}m diameter), Emiliania huxleyi (5 {\textmu}m), Coscinodiscus radiatus (50 {\textmu}m) and C. wailesii (100 {\textmu}m) are 0.11 {\textpm} 0.07, 0.20 {\textpm} 0.09, 0.41 {\textpm} 0.04 and 0.15 {\textpm} 0.20 (mean {\textpm} SD) higher than bulk seawater (pH 8.00), respectively. Thickness of the pH boundary layer of C. wailesii increases from 18 {\textpm} 4 to 122 {\textpm} 17 {\textmu}m when bulk seawater pH decreases from 8.00 to 7.78. Phycosphere pH is regulated by photosynthesis and extracellular enzymatic transformation of bicarbonate, as well as being influenced by light intensity and seawater pH and buffering capacity. The pH change alters Fe speciation in the phycosphere, and hence Fe availability to phytoplankton is likely better predicted by the phycosphere, rather than bulk seawater. Overall, the precise quantification of chemical conditions in the phycosphere is crucial for assessing the sensitivity of marine phytoplankton to ongoing ocean acidification and Fe limitation in surface oceans.},
	keywords = {Biogeochemistry, Microbial biooceanography, RCC1, rcc1731, Water microbiology},
	issn = {1751-7370},
	doi = {10.1038/s41396-022-01280-1},
	url = {https://www.nature.com/articles/s41396-022-01280-1},
	author = {Liu, Fengjie and Gledhill, Martha and Tan, Qiao-Guo and Zhu, Kechen and Zhang, Qiong and Sala{\"u}n, Pascal and Tagliabue, Alessandro and Zhang, Yanjun and Weiss, Dominik and Achterberg, Eric P. and Korchev, Yuri}
}
@article {Kashiyama2019,
	title = {Taming chlorophylls by early eukaryotes underpinned algal interactions and the diversification of the eukaryotes on the oxygenated Earth},
	journal = {The ISME Journal},
	year = {2019},
	note = {Publisher: Springer US tex.mendeley-tags: RCC164,RCC22,RCC24,RCC375,RCC916},
	month = {feb},
	pages = {1},
	abstract = {Extant eukaryote ecology is primarily sustained by oxygenic photosynthesis, in which chlorophylls play essential roles. The exceptional photosensitivity of chlorophylls allows them to harvest solar energy for photosynthesis, but on the other hand, they also generate cytotoxic reactive oxygen species. A risk of such phototoxicity of the chlorophyll must become particularly prominent upon dynamic cellular interactions that potentially disrupt the mechanisms that are designed to quench photoexcited chlorophylls in the phototrophic cells. Extensive examination of a wide variety of phagotrophic, parasitic, and phototrophic microeukaryotes demonstrates that a catabolic process that converts chlorophylls into nonphotosensitive 132,173-cyclopheophorbide enols (CPEs) is phylogenetically ubiquitous among extant eukaryotes. The accumulation of CPEs is identified in phagotrophic algivores belonging to virtually all major eukaryotic assemblages with the exception of Archaeplastida, in which no algivorous species have been reported. In addition, accumulation of CPEs is revealed to be common among phototrophic microeukaryotes (i.e., microalgae) along with dismantling of their secondary chloroplasts. Thus, we infer that CPE-accumulating chlorophyll catabolism (CACC) primarily evolved among algivorous microeukaryotes to detoxify chlorophylls in an early stage of their evolution. Subsequently, it also underpinned photosynthetic endosymbiosis by securing close interactions with photosynthetic machinery containing abundant chlorophylls, which led to the acquisition of secondary chloroplasts. Our results strongly suggest that CACC, which allowed the consumption of oxygenic primary producers, ultimately permitted the successful radiation of the eukaryotes throughout and after the late Proterozoic global oxygenation.},
	keywords = {Biochemistry, Biogeochemistry, Cellular microbiology, microbial ecology, RCC164, RCC22, RCC24, RCC375, RCC916},
	issn = {1751-7362},
	doi = {10.1038/s41396-019-0377-0},
	url = {http://www.nature.com/articles/s41396-019-0377-0},
	author = {Kashiyama, Yuichiro and Yokoyama, Akiko and Shiratori, Takashi and Hess, Sebastian and Not, Fabrice and Bachy, Charles and Gutierrez-Rodriguez, Andres and Kawahara, Jun and Suzaki, Toshinobu and Nakazawa, Masami and Ishikawa, Takahiro and Maruyama, Moe and Wang, Mengyun and Chen, Man and Gong, Yingchun and Seto, Kensuke and Kagami, Maiko and Hamamoto, Yoko and Honda, Daiske and Umetani, Takahiro and Shihongi, Akira and Kayama, Motoki and Matsuda, Toshiki and Taira, Junya and Yabuki, Akinori and Tsuchiya, Masashi and Hirakawa, Yoshihisa and Kawaguchi, Akane and Nomura, Mami and Nakamura, Atsushi and Namba, Noriaki and Matsumoto, Mitsufumi and Tanaka, Tsuyoshi and Yoshino, Tomoko and Higuchi, Rina and Yamamoto, Akihiro and Maruyama, Tadanobu and Yamaguchi, Aika and Uzuka, Akihiro and Miyagishima, Shinya and Tanifuji, Goro and Kawachi, Masanobu and Kinoshita, Yusuke and Tamiaki, Hitoshi}
}
