@article {de_la_broise_scale-up_2022,
	title = {Scale-Up to Pilot of a Non-Axenic Culture of Thraustochytrids Using Digestate from Methanization as Nitrogen Source},
	journal = {Marine Drugs},
	volume = {20},
	number = {8},
	year = {2022},
	month = {aug},
	pages = {499},
	abstract = {The production of non-fish based docosahexaenoic acid (DHA) for feed and food has become a critical need in our global context of over-fishing. The industrial-scale production of DHA{\textendash}rich Thraustochytrids could be an alternative, if costs turned out to be competitive. In order to reduce production costs, this study addresses the feasibility of the non-axenic (non-sterile) cultivation of Aurantiochytrium mangrovei on industrial substrates (as nitrogen and mineral sources and glucose syrup as carbon and energy sources), and its scale-up from laboratory (250 mL) to 500 L cultures. Pilot-scale reactors were airlift cylinders. Batch and fed-batch cultures were tested. Cultures over 38 to 62 h achieved a dry cell weight productivity of 3.3 to 5.5 g.L-1.day-1, and a substrate to biomass yield of up to 0.3. DHA productivity ranged from 10 to 0.18 mg.L-1.day-1. Biomass productivity appears linearly related to oxygen transfer rate. Bacterial contamination of cultures was low enough to avoid impacts on fatty acid composition of the biomass. A specific work on microbial risks assessment (in supplementary files) showed that the biomass can be securely used as feed. However, to date, there is a law void in EU legislation regarding the recycling of nitrogen from digestate from animal waste for microalgae biomass and its usage in animal feed. Overall, the proposed process appears similar to the industrial yeast production process (non-axenic heterotrophic process, dissolved oxygen supply limiting growth, similar cell size). Such similarity could help in further industrial developments.},
	keywords = {RCC893},
	issn = {1660-3397},
	doi = {10.3390/md20080499},
	url = {https://www.mdpi.com/1660-3397/20/8/499},
	author = {de la Broise, Denis and Ventura, Mariana and Chauchat, Luc and Guerreiro, Maurean and Michez, Teo and Vinet, Thibaud and Gautron, Nicolas and Le Grand, Fabienne and Bideau, Antoine and Go{\"\i}c, Nelly Le and Bidault, Adeline and Lambert, Christophe and Soudant, Philippe}
}
@article {russo_production_2021,
	title = {Production of Omega-3 Oil by Aurantiochytrium mangrovei Using Spent Osmotic Solution from Candied Fruit Industry as Sole Organic Carbon Source},
	journal = {Processes},
	volume = {9},
	number = {10},
	year = {2021},
	note = {Number: 10 Publisher: Multidisciplinary Digital Publishing Institute},
	month = {oct},
	pages = {1834},
	abstract = {Osmotic dehydration is an important phase in the production of dried products, including most fruits and vegetables, in the food industry. The drying process for candied fruit produces a liquid waste called {\textquotedblleft}spent osmotic solution{\textquotedblright}, which is characterized by a high content of organic compounds, mostly dissolved sugars. The sugar content of this food by-product could be valorized through the growth of biomass with a high added value. In this study, the spent osmotic solution from the candied fruit industry was used as an organic carbon source for the growth and production of docosahexaenoic acid (DHA) in the cultivation of Aurantiochytrium mangrovei RCC893. The carbon content of the standard media was completely replaced by the sugars present in this food by-product. After that, the growth condition of this strain was optimized through response surface methodologies using a central composite design (CCD), and the optimal combination of the spent osmotic solution and nitrogen was established. Moreover, a scale-up trial was performed using the optimal conditions obtained after CCD to evaluate the scalability of the process.},
	keywords = {DHA, food waste, Microalgae, PUFA, rcc, RCC893, sustainability},
	doi = {10.3390/pr9101834},
	url = {https://www.mdpi.com/2227-9717/9/10/1834},
	author = {Russo, Giovanni L. and Langellotti, Antonio L. and Blasco, Thierry and Oliviero, Maria and Sacchi, Raffaele and Masi, Paolo}
}
@article {Pollard2020,
	title = {A tunable 3D printed microfluidic resistive pulse sensor for the characterisation of algae and microplastics},
	journal = {ChemRxiv},
	year = {2020},
	note = {tex.mendeley-tags: RCC5374,RCC893},
	abstract = {Technologies that can detect and characterise particulates in liquids have applications in health, food and environmental monitoring. Here we present a low-cost and high-throughput multiuse counter that classifies a particle{\textquoteright}s size, concentration, porosity and shape. Using an additive manufacturing process, we have assembled a reusable flow resistive pulse sensor. The device remains stable for several days with repeat measurements. We demonstrate its use for characterising algae with spherical and rod structures as well as microplastics shed from teabags. We present a methodology that results in a specific signal for microplastics, namely a conductive pulse, in contrast to particles with smooth surfaces such as calibration particles or algae, allowing the presence of microplastics to be easily confirmed and quantified. In addition, the shape of the signal and particle are correlated, giving an extra physical property to characterise suspended particulates. The technology can rapidly screen volumes of liquid, 1 mL/ min, for the presence of microplastics and algae.},
	keywords = {RCC5374, RCC893},
	issn = {2187-4247},
	doi = {10.26434/chemrxiv.12249833.v1},
	author = {Pollard, M. and Hunsicker, E. and Platt, M.}
}
@article {Raghu-Kumar1988,
	title = {Schizochytrium mangrovei sp. nov., a thraustochytrid from mangroves in India},
	journal = {Transactions of the British Mycological Society},
	volume = {90},
	number = {4},
	year = {1988},
	note = {ISBN: 0007-1536 Publisher: British Mycological Society tex.mendeley-tags: RCC893},
	month = {jun},
	pages = {627{\textendash}631},
	abstract = {Schizochytrium mangrovei sp. nov. on decaying mangrove leaves is described from Goa, India. In sea water/pine pollen cultures, the encysted zoospore divides by repeated binary divisions to form 4, 6, 8 or 12 cells. Instead of forming sporangia as in the other species of Schizochytrium, these cells directly become zoospores. In nutrient liquid media, the developmental stages resemble those of Ulkenia. A key to the species of Schizochytrium is given.},
	keywords = {RCC893},
	issn = {00071536},
	doi = {10.1016/S0007-1536(88)80068-8},
	url = {http://linkinghub.elsevier.com/retrieve/pii/S0007153688800688},
	author = {Raghu-Kumar, S.}
}
