%0 Journal Article %J Phycologia %D 2017 %T Rediscovery of the Ochromonas type species Ochromonas triangulata (Chrysophyceae) from its type locality (Lake Veysove, Donetsk region, Ukraine) %A Andersen, Robert A. %A Graf, Louis %A Malakhov, Yuriy %A Yoon, Hwan Su %K 2017 %K RCC21 %X Ochromonas triangulata, the type species for a genus with over 125 taxa, was collected for only the second time, again from the type locality. Cell morphology, cell division, palmelloid stage and cyst structure generally agreed with the original description. Molecular phylogenetic analysis based on the 18S rRNA gene revealed 13 clades of Ochromonas-like flagellates as well as the clade represented by our O. triangulata strain and the nearly identical strain RCC-21/AC025. We also conducted a concatenated analysis using the 18S rRNA and the rbcL genes, and we recovered the same 14 clades. One clade, containing strains CCAP 933/27 and CCMP1861, previously named Ochromonas tuberculata, was re-identified as Chrysastrella paradoxa and Chrysastrella breviappendiculata, respectively. One clade included the Poterioochromonas strains but we were unable to convincingly connect species names to the strains because authentic strains were unknown or not examined. Organisms in the clade that included the well-known Ochromonas danica were assigned to Chlorochromonas. The generic name Melkoniania gen. nov. was proposed for one distinct clade of marine flagellates. These changes addressed some of the issues associated with the polyphyletic Ochromonas sensu lato but many problems remained. %B Phycologia %V 56 %P 591–604 %G eng %U http://www.phycologia.org/doi/10.2216/17-15.1 %R 10.2216/17-15.1 %0 Journal Article %J European Journal of Phycology %D 2015 %T Phaeocystis rex sp. nov. (Phaeocystales, Prymnesiophyceae): a new solitary species that produces a multilayered scale cell covering %A Andersen, Robert A. %A Bailey, J. Craig %A Decelle, Johan %A Probert, Ian %K 2015 %K algae %K organic scales %K Phaeocystales %K Phaeocystis rex %K Prymnesiophyceae %K rcc %K RCC4025 %K RCC?o?dd %K SBR$_\textrmP$hyto$_\textrmE$PPO %K systematics %K ultrastructure %X A morphologically distinct marine species, Phaeocystis rex sp. nov., was described on the basis of light microscopy, transmission electron microscopy and DNA sequence comparisons. Non-motile cells were solitary (non-colonial), 6–10 µm in diameter and 8–15 µm long, and possessed chloroplasts with distinctive finger-like lobes. TEM observations demonstrated the presence of two short flagella and a very short haptonema that arose from an invagination of the protoplast. Non-motile cells were surrounded by one to several dense layers composed of scales, presumably unmineralized, and an amorphous material. Phylogenetic analyses based upon combined partial nucleotide sequences for five nuclear-or plastid-encoded genes (18S rRNA, 28S rRNA, 16S rRNA, psbA and rbcL) from cultured strains and from uncharacterized acantharian symbionts confirmed that P. rex was a distinct species. These analyses implied that P. rex occupies an intermediate evolutionary position between solitary and colonial Phaeocystis species. %B European Journal of Phycology %V 50 %P 207–222 %G eng %U http://www.tandfonline.com/doi/full/10.1080/09670262.2015.1024287 %R 10.1080/09670262.2015.1024287