@article {fan_quantifying_2022, title = {Quantifying the Extent of Calcification of a Coccolithophore Using a Coulter Counter}, journal = {Analytical Chemistry}, year = {2022}, month = {sep}, pages = {acs.analchem.2c01971}, abstract = {Although, in principle, the Coulter Counter technique yields an absolute measure of particle volume, in practice, calibration is nearuniversally employed. For regularly shaped and non-biological samples, the use of latex beads for calibration can provide sufficient accuracy. However, this is not the case with particles encased in biogenically formed calcite. To date, there has been no effective route by which a Coulter Counter can be calibrated to enable the calcification of coccolithophores�single cells encrusted with biogenic calcite�to be quantified. Consequently, herein, we seek to answer the following question: to what extent can a Coulter Counter be used to provide accurate information regarding the calcite content of a singlespecies coccolithophore population? Through the development of a new calibration methodology, based on the measurement and dynamic tracking of the acid-driven calcite dissolution reaction, a route by which the cellular calcite content can be determined is presented. This new method allows, for the first time, a Coulter Counter to be used to yield an absolute measurement of the amount of calcite per cell.}, keywords = {RCC1198, RCC1216, RCC1314}, issn = {0003-2700, 1520-6882}, doi = {10.1021/acs.analchem.2c01971}, url = {https://pubs.acs.org/doi/10.1021/acs.analchem.2c01971}, author = {Fan, Xinmeng and Batchelor-McAuley, Christopher and Yang, Minjun and Barton, Samuel and Rickaby, Rosalind E. M. and Bouman, Heather A. and Compton, Richard G.} } @article {Barton2019, title = {Quantifying the temperature dependence of growth rate in marine phytoplankton within and across species}, journal = {Limnology and Oceanography}, year = {2019}, note = {tex.mendeley-tags: RCC1303,RCC1512,RCC1731,RCC1773,RCC4221,RCC539,RCC6,RCC623,RCC626,RCC652,RCC653,RCC655,RCC834,RCC88}, abstract = {Abstract Models of marine biogeochemistry capture the effects of temperature on phytoplankton growth via the monotonic, exponential Eppley coefficient, without considering the physiological or evolutionary processes that underpin this emergent, across-species temperature response. Here, we investigated both the within- and across-species temperature dependence of growth rate for 18 species of marine phytoplankton. We found that the temperature dependence of growth rate derived across species was lower than the average temperature response within species. This finding supports a {\textquotedblleft}partial compensation{\textquotedblright} model of thermal adaptation and suggests that adaptation can partially compensate for the underlying thermodynamic effects of temperature on physiological rates observed within species. We also found that thermal tolerance traits (e.g. the optimum temperature for growth) systematically covaried with a host of key functional traits (e.g. cell size, elemental composition). Consequently, turnover in species composition in a warmer ocean, linked to interspecific variability in thermal tolerance traits, could be associated with major shifts in the functional trait composition of marine phytoplankton communities with far reaching implications for ecosystem functioning.}, keywords = {RCC1303, rcc1512, rcc1731, RCC1773, RCC4221, rcc539, RCC6, RCC623, RCC626, RCC652, RCC653, RCC655, RCC834, RCC88}, issn = {0024-3590}, doi = {10.1002/lno.11170}, author = {Barton, Samuel and Yvon-Durocher, Gabriel} } @article {Limardo2017, title = {Quantitative biogeography of picoprasinophytes establishes ecotype distributions and significant contributions to marine phytoplankton}, journal = {Environmental Microbiology}, year = {2017}, note = {tex.mendeley-tags: RCC1105,RCC715,RCC716,RCC809}, month = {jun}, keywords = {RCC1105, RCC715, RCC716, RCC809}, issn = {14622912}, doi = {10.1111/1462-2920.13812}, url = {http://doi.wiley.com/10.1111/1462-2920.13812}, author = {Limardo, Alexander J. and Sudek, Sebastian and Choi, Chang Jae and Poirier, Camille and Rii, Yoshimi M. and Blum, Marguerite and Roth, Robyn and Goodenough, Ursula and Church, Matthew J. and Worden, Alexandra Z.} } @article {Dominguez-Martin2017, title = {Quantitative proteomics shows extensive remodeling induced by nitrogen limitation in prochlorococcus marinus SS120}, journal = {mSystems}, volume = {2}, number = {3}, year = {2017}, note = {tex.mendeley-tags: RCC156}, month = {jun}, pages = {e00008{\textendash}17}, abstract = {Prochlorococcus requires the capability to accommodate to environmental changes in order to proliferate in oligotrophic oceans, in particular regarding nitrogen availability. A precise knowledge of the composition and changes in the proteome can yield fundamental insights into such a response. Here we report a detailed proteome analysis of the important model cyanobacterium Prochlorococcus marinus SS120 after treatment with azaserine, an inhibitor of ferredoxin-dependent glutamate synthase (GOGAT), to simulate extreme nitrogen starvation. In total, 1,072 proteins, corresponding to 57\% of the theoretical proteome, were identified{\textemdash}the maximum proteome coverage obtained for any Prochlorococcus strain thus far. Spectral intensity, calibrated quantification by the Hi3 method, was obtained for 1,007 proteins. Statistically significant changes ( P value of {\textexclamdown}0.05) were observed for 408 proteins, with the majority of proteins (92.4\%) downregulated after 8 h of treatment. There was a strong decrease in ribosomal proteins upon azaserine addition, while many transporters were increased. The regulatory proteins P II and PipX were decreased, and the global nitrogen regulator NtcA was upregulated. Furthermore, our data for Prochlorococcus indicate that NtcA also participates in the regulation of photosynthesis. Prochlorococcus responds to the lack of nitrogen by slowing down translation, while inducing photosynthetic cyclic electron flow and biosynthesis of proteins involved in nitrogen uptake and assimilation.}, keywords = {RCC156}, issn = {2379-5077}, doi = {10.1128/mSystems.00008-17}, url = {http://msystems.asm.org/lookup/doi/10.1128/mSystems.00008-17}, author = {Dom{\'\i}nguez-Mart{\'\i}n, Maria Agustina and G{\'o}mez-Baena, Guadalupe and D{\'\i}ez, Jes{\'u}s and L{\'o}pez-Grueso, Maria Jos{\'e} and Beynon, Robert J. and Garc{\'\i}a-Fern{\'a}ndez, Jos{\'e} Manuel}, editor = {Huber, Julie A.} } @article {Biegala2003, title = {Quantitative assessment of picoeucaryotes in the natural environment using taxon specific oligonucleotide probes in association with TSA-FISH (Tyramide Signal Amplification - Fluorescent In Situ Hybridization) and flow cytometry}, journal = {Applied and Environmental Microbiology}, volume = {69}, year = {2003}, note = {tex.mendeley-tags: 2003,rcc,sbr?hyto}, pages = {5519{\textendash}5529}, keywords = {2003, PICODIV, rcc, SBR$_\textrmP$hyto, sbr?hyto, SOMLIT}, doi = {10.1128/AEM.69.9.5519-5529.2003}, author = {Biegala, I C and Not, F and Vaulot, D and Simon, N} }