@article {Baudoux2015, title = {Interplay between the genetic clades of {\textexclamdown}i{\textquestiondown}Micromonas{\textexclamdown}/i{\textquestiondown} and their viruses in the Western English Channel.}, journal = {Environmental microbiology reports}, year = {2015}, note = {tex.mendeley-tags: 2015,rcc,sbr?hyto$_\textrmd$ipo}, month = {jun}, abstract = {The genus Micromonas comprises distinct genetic clades that commonly dominate eukaryotic phytoplankton community from polar to tropical waters. This phytoplankter is also recurrently infected by abundant and genetically diverse prasinoviruses. Here we report on the interplay between prasinoviruses and Micromonas with regards to the genetic diversity of this host. During one year, we monitored the abundance of 3 clades of Micromonas and their viruses in the Western English Channel both in the environment, using clade-specific probes and flow cytometry, and in the laboratory, using clonal strains of Micromonas clades to assay for their viruses by plaque-forming units. We showed that the seasonal fluctuations of Micromonas clades were closely mirrored by the abundances of their corresponding viruses, indicating that the members of Micromonas genus are susceptible to viral infection, regardless of their genetic affiliation. The characterization of 45 viral isolates revealed that Micromonas clades are attacked by specific virus populations, which exhibit distinctive clade specificity, life strategies, and genetic diversity. However, some viruses can also cross-infect different host clades suggesting a mechanism of horizontal gene transfer within Micromonas genus. This study provides novel insights into the impact of viral infection for the ecology and evolution of the prominent phytoplankter Micromonas.}, keywords = {2015, rcc, RCC?o?dd, sbr?hyto$_\textrmd$ipo}, issn = {1758-2229}, doi = {10.1111/1758-2229.12309}, url = {http://www.ncbi.nlm.nih.gov/pubmed/26081716}, author = {Baudoux, A-C and Lebredonchel, H and Dehmer, H and Latimier, M and Edern, R and Rigaut-Jalabert, F and Ge, P and Guillou, L and Foulon, E and Bozec, Y and Cariou, T and Desdevises, Y and Derelle, E and Grimsley, N and Moreau, H and Simon, N} } @article {Foulon2008, title = {Ecological niche partitioning in the picoplanktonic green alga Micromonas pusilla: evidence from environmental surveys using phylogenetic probes}, journal = {Environmental Microbiology}, volume = {10}, year = {2008}, note = {tex.mendeley-tags: 2008,rcc,sbr?hyto$_\textrmd$ipo}, pages = {2433{\textendash}2443}, keywords = {2008, rcc, SBR$_\textrmP$hyto$_\textrmD$PO, sbr?hyto$_\textrmd$ipo}, doi = {10.1111/j.1462-2920.2008.01673.x}, author = {Foulon, E and Not, F and Jalabert, F and Cariou, T and Massana, R and Simon, N} } @inbook {Marie2005, title = {Phytoplankton cell counting by flow cytometry}, booktitle = {Algal culturing techniques}, year = {2005}, note = {tex.mendeley-tags: 2005,rcc,sbr?hyto}, pages = {253{\textendash}267}, publisher = {Academic Press}, organization = {Academic Press}, keywords = {2005, PICODIV, rcc, SBR$_\textrmP$hyto$_\textrmD$PO, sbr?hyto}, author = {Marie, D and Simon, N and Vaulot, D}, editor = {Andersen, R A} } @article {Biegala2003, title = {Quantitative assessment of picoeucaryotes in the natural environment using taxon specific oligonucleotide probes in association with TSA-FISH (Tyramide Signal Amplification - Fluorescent In Situ Hybridization) and flow cytometry}, journal = {Applied and Environmental Microbiology}, volume = {69}, year = {2003}, note = {tex.mendeley-tags: 2003,rcc,sbr?hyto}, pages = {5519{\textendash}5529}, keywords = {2003, PICODIV, rcc, SBR$_\textrmP$hyto, sbr?hyto, SOMLIT}, doi = {10.1128/AEM.69.9.5519-5529.2003}, author = {Biegala, I C and Not, F and Vaulot, D and Simon, N} } @article {Not2002, title = {Application of fluorescent in situ hybridization coupled with tyramide signal amplification (FISH-TSA) to assess eukaryotic picoplankton composition}, volume = {28}, year = {2002}, note = {Publication Title: Aquatic microbial ecology tex.mendeley-tags: RCC,rcc}, pages = {157{\textendash}166}, abstract = {Photosynthetic picoeukaryotes (phytoplankton cells with a diameter smaller than 2 to 3 ??m) contribute significantly to both biomass and primary production in the oligotrophic open ocean and coastal waters, at certain times of the year. The identification of these organisms is difficult because of their small size and simple morphology, therefore hindering detailed ecological studies of their distribution and role. In this paper, we demonstrate the use of oligonucleotide probes specific to algal classes or to lower order taxa in combination with fluorescent in situ hybridization and tyramide signal amplification (FISH-TSA) to determine eukaryotic picophytoplankton diversity. Target cells were detected and enumerated using epifluorescence microscopy. The sensitivity of the technique and the specificity of the probes were tested on pure and mixed picoplanktonic strains, as well as on natural samples from the English Channel. In these samples, the community was dominated by cells belonging to the division Chlorophyta. Haptophyta, Bolidophyceae and Pelagophyceae were also detected at low abundance. The FISH-TSA method is readily applicable to the study of picoplankton diversity in natural communities.}, keywords = {rcc}, issn = {0948-3055}, doi = {10.3354/ame028157}, author = {Not, F and Simon, N and Biegala, IC and Vaulot, D} } @article {Simon1994, title = {Characterization of oceanic photosynthetic picoeukaryotes by flow cytometry analysis}, journal = {Journal of Phycology}, volume = {30}, year = {1994}, note = {tex.mendeley-tags: RCC,rcc}, pages = {922{\textendash}935}, keywords = {flow cytometry, hplc, pigments, rcc, RCC SBR$_\textrmP$hyto, \#PICOPLANKTON}, doi = {10.1111/j.0022-3646.1994.00922.x}, author = {Simon, N and Barlow, R G and Marie, D and Partensky, F and Vaulot, D} }