@article {uwizeye_morphological_2021, title = {Morphological bases of phytoplankton energy management and physiological responses unveiled by 3D subcellular imaging}, journal = {Nature Communications}, volume = {12}, number = {1}, year = {2021}, note = {Number: 1 Publisher: Nature Publishing Group}, month = {feb}, pages = {1{\textendash}12}, abstract = {Phytoplankton account for a large proportion of global primary production and comprise a number of phylogenetically distinct lineages. Here, Uwizeye et al. use FIB-SEM to study ultrastructural plasticity of 7 distinct taxa and describe how subcellular organisation is linked to energy metabolism.}, keywords = {RCC100, RCC4014, RCC827, RCC909}, issn = {2041-1723}, doi = {10.1038/s41467-021-21314-0}, url = {http://www.nature.com/articles/s41467-021-21314-0}, author = {Uwizeye, Clarisse and Decelle, Johan and Jouneau, Pierre-Henri and Flori, Serena and Gallet, Benoit and Keck, Jean-baptiste and Bo, Davide Dal and Moriscot, Christine and Seydoux, Claire and Chevalier, Fabien and Schieber, Nicole L. and Templin, Rachel and Allorent, Guillaume and Courtois, Florence and Curien, Gilles and Schwab, Yannick and Schoehn, Guy and Zeeman, Samuel C. and Falconet, Denis and Finazzi, Giovanni} } @article {Uwizeye2020, title = {In-cell quantitative structural imaging of phytoplankton using 3D electron microscopy}, journal = {bioRxiv}, year = {2020}, note = {tex.mendeley-tags: RCC100,RCC4014,RCC827,RCC909}, month = {jan}, pages = {2020.05.19.104166}, abstract = {Phytoplankton is a minor fraction of the global biomass playing a major role in primary production and climate. Despite improved understanding of phytoplankton diversity and genomics, we lack nanoscale subcellular imaging approaches to understand their physiology and cell biology. Here, we present a complete Focused Ion Beam - Scanning Electron Microscopy (FIB-SEM) workflow (from sample preparation to image processing) to generate nanometric 3D phytoplankton models. Tomograms of entire cells, representatives of six ecologically-successful phytoplankton unicellular eukaryotes, were used for quantitative morphometric analysis. Besides lineage-specific cellular architectures, we observed common features related to cellular energy management: i) conserved cell-volume fractions occupied by the different organelles; ii) consistent plastid-mitochondria interactions, iii) constant volumetric ratios in these energy-producing organelles. We revealed detailed subcellular features related to chromatin organization and to biomineralization. Overall, this approach opens new perspectives to study phytoplankton acclimation responses to abiotic and biotic factors at a relevant biological scale.Competing Interest StatementThe authors have declared no competing interest.}, keywords = {RCC100, RCC4014, RCC827, RCC909}, doi = {10.1101/2020.05.19.104166}, url = {http://biorxiv.org/content/early/2020/05/20/2020.05.19.104166.abstract}, author = {Uwizeye, Clarisse and Decelle, Johan and Jouneau, Pierre-Henri and Gallet, Benoit and Keck, Jean-baptiste and Schwab, Yannick and Schoehn, Guy and Zeeman, Samuel C and Falconet, Denis and Finazzi, Giovanni and Moriscot, Christine and Chevalier, Fabien and Schieber, Nicole L and Templin, Rachel and Curien, Gilles and Schwab, Yannick and Schoehn, Guy and Zeeman, Samuel C and Falconet, Denis and Finazzi, Giovanni} }