@article {das_assessing_2023,
	title = {Assessing indicators of arsenic toxicity using variable fluorescence in a commercially valuable microalgae: physiological and toxicological aspects},
	journal = {Journal of Hazardous Materials},
	year = {2023},
	month = {mar},
	pages = {131215},
	abstract = {Indicators signaling Arsenic (As) stress through physiology of microalgae using non-destructive methods like variable fluorescence are rare but requisite. This study reports stress markers indicating arsenic (As) toxicity (in two concentrations 11.25{\textmu}g/L and 22.5{\textmu}g/L compared to a control) exposed to a microalga (Diacronema lutheri), using fast repetition rate fluorometry (FRRf). Growth and physiological parameters such as cell density, chl a and the maximum quantum yield Fv/Fm showed coherence and impeded after the exponential phase (day 9 - day 12) in As treatments compared to the control (p < 0.05). On contrary photo-physiological constants were elevated showing higher optical (aLHII) and functional [Sigma (σPSII)] absorption cross-section for the As treatments (p < 0.05) further implying the lack of biomass production yet an increase in light absorption. In addition, As exposure increased the energy dissipation by heat (NPQ-NSV) showing a strong relationship with the de-epoxidation ratio (DR) involving photoprotective pigments. Total As bioaccumulation by D. lutheri showed a strong affinity with Fe adsorption throughout the algal growth curve. This study suggests some prompt photo-physiological proxies signaling As contamination and endorsing its usefulness in risk assessments, given the high toxicity and ubiquitous presence of As in the ecosystem.},
	keywords = {Arsenic bioaccumulation, de-epoxidation ratio (DR), Optical absorption cross section, pigment concentrations, RCC1537},
	issn = {0304-3894},
	doi = {10.1016/j.jhazmat.2023.131215},
	url = {https://www.sciencedirect.com/science/article/pii/S0304389423004971},
	author = {Das, Shagnika and Lizon, Fabrice and Gevaert, Fran{\c c}ois and Bialais, Capucine and Duong, Gwendoline and Ouddane, Baghdad and Souissi, Sami}
}
@article {das_trace_2022,
	title = {Trace metals exposure in three different coastal compartments show specific morphological and reproductive traits across generations in a sentinel copepod},
	journal = {Science of The Total Environment},
	year = {2022},
	month = {nov},
	pages = {160378},
	abstract = {The effect of exposure from several compartments of the environment at the level of individuals were rarely investigated. This study reports the effect of contaminants from varied compartments like sediment resuspension, elutriation from resuspended sediment (extract) and seawater spiked trace metal mixtures (TM) on morphological and reproductive traits of the pelagic bioindicator copepod Eurytemora affinis. At the population level of E. affinis, lowest survival was observed in dissolved exposures (TM and extract) in the first generation (G1), showing some adaptation in the second generation (G2). An opposite trend for resuspended sediment showed higher sensitivity in survival at G2. At the individual level, prosome length and volume proved to be sensitive parameters for resuspended sediments, whereas clutch size and egg diameter were more sensitive to TM and extract. Although the generation of decontamination (G3, no exposure), showed a significant recovery at the population level (survival \% along with clutch size) of E. affinis exposed to resuspended sediment, morphological characteristics like prosome length and volume showed no such recovery (lower than control, p <0.05). To the contrary, dissolved exposure showed no significant recovery from G1 to G3 on neither survival \%, clutch size, egg diameter, prosome volume, but an increase of prosome length (p <0.05). Such tradeoffs in combatting the stress from varied sources of toxicity was observed in all exposures, from G1 to G3. The number of lipid droplets inside the body cavity of E. affinis showed a significant positive correlation with trace metal bioaccumulation (p <0.01) along with a negative correlation (p <0.05) with survival and clutch size in each treatment. This confirms the inability of copepods to utilize lipids under stressful conditions. Our study tenders certain morphological and reproductive markers that show specificity to different compartments of exposure, promising an advantage in risk assessment and fish feed studies.},
	keywords = {RCC1537},
	issn = {00489697},
	doi = {10.1016/j.scitotenv.2022.160378},
	url = {https://linkinghub.elsevier.com/retrieve/pii/S0048969722074800},
	author = {Das, Shagnika and Souissi, Anissa and Ouddane, Baghdad and Hwang, Jiang-Shiou and Souissi, Sami}
}
@article {pan_effects_2021,
	title = {Effects of Epibiotic Diatoms on the Productivity of the Calanoid Copepod Acartia tonsa (Dana) in Intensive Aquaculture Systems},
	journal = {Frontiers in Marine Science},
	volume = {8},
	year = {2021},
	pages = {1386},
	abstract = {We evaluated here the effects of the epibiotic diatom Tabularia sp. on the productivity of the calanoid copepod Acartia tonsa (Dana) for assessing their risk on copepod intensive aquaculture industry for the provision of live feed. In the first experiment, uninfested and intensively infested females were cultivated individually for the assessment of egg production. Intensively infested females appeared to have a significantly lower egg production (5.0{\textendash}9.0 eggs/female/d) than uninfested females (22.0{\textendash}26.0 eggs/female/d) during 5 consecutive days. In the second experiment, effects of culture densities on diatom epibiosis were investigated in 9 L cultures at three different densities (200, 400, and 600 ind. L{\textendash}1). Another culture at higher volume (250 L) and lowest density (200 ind. L{\textendash}1) was also carried out to test the effect of culture volume on diatom epibiosis. The infestation rate (\%), infestation intensity (ratio of surface diatom coverage levels, classified as levels 0{\textendash}3) and daily egg harvest rate (number of harvested eggs per day per liter) were evaluated among the four culture populations. The copepods had higher infestation rate (53.69{\textendash}60.14\%) and intensity rate (high ratios at level 2 and 3) when the densities were increased from 200 ind./L to 400 and 600 ind./L. Although egg harvest increased with increasing culture density, it seemed that the diatom-infested A. tonsa population reach a saturated egg production when the density was higher than 400 ind./L. Nevertheless, the differences of culture volumes (250 and 9 L) appeared to be not to have any effect when the copepods were cultivated at the same density (200 ind./L). This study reveals for the first time that the epibiosis of the diatom Tabularia sp. reduces the individual egg production, and egg harvest rate in high-density culture of the copepod A. tonsa. Our findings implicate that diatom epibiosis should be avoid in copepod intensive culture systems.},
	keywords = {rcc, RCC350},
	issn = {2296-7745},
	doi = {10.3389/fmars.2021.728779},
	url = {https://www.frontiersin.org/article/10.3389/fmars.2021.728779},
	author = {Pan, Yen-Ju and Wang, Wei-Lung and Hwang, Jiang-Shiou and Souissi, Sami}
}
@article {dayras_microalgal_2021,
	title = {Microalgal Diet Influences the Nutritive Quality and Reproductive Investment of the Cyclopoid Copepod Paracyclopina nana},
	journal = {Frontiers in Marine Science},
	volume = {8},
	year = {2021},
	pages = {1147},
	abstract = {Copepods represent an interesting alternative or a complement live food to brine shrimps and rotifers commonly used in aquaculture. They constitute the natural prey of many fish species and therefore do not require a potential nutritional enrichment. But an optimization of the microalgal diets used to feed copepods is essential to improve their mass culture. This study examined the effects of seven microalgal diets, namely single-species diets of Rhodomonas salina (R), Tisochrysis lutea (T), and Pavlova lutheri (=Diacronema lutheri) (P), two-species diets (R + T, T + P, and R + P), and a three-species diet (R + T + P), on the fatty acid and monosaccharide composition of the cyclopoid copepod Paracyclopina nana as well as its reproductive investment. Experiments were run during 15 days in 10-L beakers; starting with nauplii collected from a large 300-L batch culture. Copepods fatty acid contents were studied, particularly the relative amounts of docosahexaenoic acid (DHA) and eicosa-pentaenoic acid (EPA). The R + T, R, and T diets induced the highest total fatty acid amount in copepods. R + T and R also generated the lowest DHA/EPA ratios in copepods due to high EPA contents. The highest value of total monosaccharides was found in copepods fed with R + T + P. Diets R + T and R induced the greatest prosome volumes and clutch volumes in ovigerous females. Both prosome volume and clutch volume in P. nana ovigerous females were correlated to the individual EPA amount. The results demonstrated that all diets including R. salina enhanced the productivity of P. nana in mass culture, particularly when combined with T. lutea. R. salina, and T. lutea induced complementary fatty acid and monosaccharide profiles, confirming that R + T represents the best microalgae combination for productive culture of P. nana. Conversely, P. lutheri did not enhance the nutritional profile nor the fecundity of P. nana in the culture. This study is the first to demonstrate that R. salina is a suitable microalga for productive mass culture of P. nana for use as live food in aquaculture.},
	keywords = {rcc, RCC1349, RCC1537, RCC20},
	issn = {2296-7745},
	doi = {10.3389/fmars.2021.697561},
	url = {https://www.frontiersin.org/article/10.3389/fmars.2021.697561},
	author = {Dayras, Paul and Bialais, Capucine and Sadovskaya, Irina and Lee, Min-Chul and Lee, Jae-Seong and Souissi, Sami}
}
@article {das_single_2021,
	title = {Single toxicity of arsenic and combined trace metal exposure to a microalga of ecological and commercial interest: Diacronema lutheri},
	journal = {Chemosphere},
	year = {2021},
	pages = {132949},
	abstract = {Eco-toxicological assays with species of economic interest such as Diacronema lutheri are essential for industries that produce aquaculture feed, natural food additives and also in drug developing industries. Our study involved the exposure of a single and combined toxicity of arsenic (As V) to D. lutheri for the entire algal growth phase and highlighted that a combined exposure of As V with other essential (Copper, Cu; Nickel, Ni) and non-essential (Cadmium, Cd; Lead, Pb) trace metals reduced significantly the cell number, chlorophyll a content, and also significantly increased the de-epoxidation ratio (DR) as a stress response when compared to the single toxicity of As V. Arsenic, as one of the ubiquitous trace metal and an active industrial effluent is reported to have an increased bio-concentration factor when in mixture with other trace metals in this study. In the combined exposure, the concentration of total As bio-accumulated by D. lutheri was higher than in the single exposure. Hence, polluted areas with the prevalence of multiple contaminants along with the highly toxic trace metals like As can impose a greater risk to the exposed organisms that may get further bio-magnified in the food chain. Our study highlights the consequences and the response of D. lutheri in terms of contamination from single and multiple trace metals in order to obtain a safer biomass production for the growing need of natural derivatives.},
	keywords = {Arsenic, bioaccumulation, Bioconcentration factor, Chlorophyll, de-epoxidation ratio, RCC1537, Trace metals mixture},
	issn = {0045-6535},
	doi = {10.1016/j.chemosphere.2021.132949},
	url = {https://www.sciencedirect.com/science/article/pii/S0045653521034214},
	author = {Das, Shagnika and Gevaert, Fran{\c c}ois and Ouddane, Baghdad and Duong, Gwendoline and Souissi, Sami}
}
@article {Pan2020,
	title = {Assessments of first feeding protocols on the larviculture of California grunion Leuresthes tenuis (Osteichthyes: Atherinopsidae)},
	journal = {Aquaculture Research},
	volume = {51},
	number = {7},
	year = {2020},
	note = {Publisher: Blackwell Publishing Ltd tex.mendeley-tags: RCC350},
	month = {jul},
	pages = {3054{\textendash}3058},
	keywords = {California grunion, Copepod, larval rearing, live feed, quiescent egg, RCC350},
	issn = {13652109},
	doi = {10.1111/are.14637},
	url = {https://onlinelibrary.wiley.com/doi/10.1111/are.14637},
	author = {Pan, Yen Ju and D{\'e}pos{\'e}, Emilien and Souissi, Anissa and H{\'e}nard, St{\'e}phane and Schaadt, Mike and Mastro, Ed and Souissi, Sami}
}
@article {Gnouma2017,
	title = {Changes in fatty acids profile, monosaccharide profile and protein content during batch growth of Isochrysis galbana (T.iso)},
	journal = {Aquaculture Research},
	volume = {48},
	number = {9},
	year = {2017},
	note = {Publisher: John Wiley \& Sons, Ltd (10.1111) tex.mendeley-tags: RCC1349},
	month = {sep},
	pages = {4982{\textendash}4990},
	abstract = {Abstract To investigate the nutritional value of the marine micro-alga Isochrysis galbana Tahitian Isochrysis strain (T.iso) as an alternative feed for aquaculture during culture age, its biochemical composition was studied under autotrophic and controlled culture conditions at different growth stages: exponential phase, early and late stationary phases and decay phase. Analysis showed that C14:0, C16:0, C16:1, C18:4 (n-3) and C22:6 (n-3) were the most abundant fatty acids in this alga at different growth stages. The highest values of monounsaturated fatty acids were recorded at the late stationary and the decay phases. However, the highest levels of polyunsaturated fatty acids were observed at the early stationary phase. At all growth stages, I. galbana (T.iso) contained arabinose, xylose, mannose, galactose and glucose. Glucose represented the main sugar, and its content per dry alga biomass weight increased with increasing age of the culture and reached about fourfold in the decay phase. The maximum protein content was also observed during this last phase.},
	keywords = {fatty acids, growth phases, Isochrysis galbana (T.iso), monosaccharides, proteins, RCC1349},
	issn = {1355-557X},
	doi = {10.1111/are.13316},
	url = {https://doi.org/10.1111/are.13316},
	author = {Gnouma, Asma and Sadovskaya, Irina and Souissi, Anissa and Sebai, Khaled and Medhioub, Amel and Grard, Thierry and Souissi, Sami}
}
@article {Arias2017,
	title = {Removal and biodegradation of phenanthrene, fluoranthene and pyrene by the marine algae rhodomonas baltica enriched from north atlantic coasts},
	journal = {Bulletin of Environmental Contamination and Toxicology},
	volume = {98},
	number = {3},
	year = {2017},
	note = {tex.mendeley-tags: RCC350},
	pages = {392{\textendash}399},
	abstract = {This study is focused on the removal, accumulation and degradation of three environmental ubiquitous polycyclic aromatic hydrocarbons (PAHs), phenanthrene (PHE), fluoranthene (FLA) and pyrene (PYR), by the marine alga Rhodomonas baltica enriched from the English Channel. After separation, purification and culture in several phases, R. baltica was exposed to PAH concentrations that are frequently encountered in the field in several anthropized environments. The results showed that R. baltica can grow under PAH stress, efficiently remove up to 70\% of these compounds from the medium by 216 h of culture and selectively bioaccumulate PAHs by their hydrophobicity. Between PHE, FLA and PYR, phenanthrene was the compound with higher degradation rates throughout incubation. The equilibrium partitioning theoretical approach showed that physico-chemical partitioning, rather than active bioconcentration, was the major factor governing the bioaccumulation, outlying a potential application in decontamination processes for this species.},
	keywords = {RCC350},
	issn = {1432-0800},
	doi = {10.1007/s00128-016-1967-4},
	url = {https://doi.org/10.1007/s00128-016-1967-4},
	author = {Arias, Andr{\'e}s H and Souissi, Anissa and Glippa, Olivier and Roussin, Marion and Dumoulin, David and Net, Sopheak and Ouddane, Baghdad and Souissi, Sami}
}
