@article {harcourt_intraspecific_2024,
	title = {Intraspecific trait variation modulates the temperature effect on elemental quotas and stoichiometry in marine Synechococcus},
	journal = {PLOS ONE},
	volume = {19},
	number = {3},
	year = {2024},
	note = {Publisher: Public Library of Science},
	month = {mar},
	pages = {e0292337},
	abstract = {Diverse phytoplankton modulate the coupling between the ocean carbon and nutrient cycles through life-history traits such as cell size, elemental quotas, and ratios. Biodiversity is mostly considered at broad functional levels, but major phytoplankton lineages are themselves highly diverse. As an example, Synechococcus is found in nearly all ocean regions, and we demonstrate contains extensive intraspecific variation. Here, we grew four closely related Synechococcus isolates in serially transferred cultures across a range of temperatures (16{\textendash}25{\textdegree}C) to quantify for the relative role of intraspecific trait variation vs. environmental change. We report differences in cell size (p<0.01) as a function of strain and clade (p<0.01). The carbon (QC), nitrogen (QN), and phosphorus (QP) cell quotas all increased with cell size. Furthermore, cell size has an inverse relationship to growth rate. Within our experimental design, temperature alone had a weak physiological effect on cell quota and elemental ratios. Instead, we find systemic intraspecific variance of C:N:P, with cell size and N:P having an inverse relationship. Our results suggest a key role for intraspecific life history traits in determining elemental quotas and stoichiometry. Thus, the extensive biodiversity harbored within many lineages may modulate the impact of environmental change on ocean biogeochemical cycles.},
	keywords = {Biogeochemistry, Cell enumeration techniques, Oceans, Particulates, Phylogenetics, phytoplankton, RCC1086, RCC2673, RCC515, Stoichiometry, Synechococcus},
	issn = {1932-6203},
	doi = {10.1371/journal.pone.0292337},
	url = {https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0292337},
	author = {Harcourt, Renne and Garcia, Nathan S. and Martiny, Adam C.}
}
@article {Garcia2018,
	title = {High variability in cellular stoichiometry of carbon, nitrogen, and phosphorus within classes of marine eukaryotic phytoplankton under sufficient nutrient conditions},
	journal = {Frontiers in Microbiology},
	volume = {9},
	number = {March},
	year = {2018},
	note = {tex.mendeley-tags: RCC103,RCC1242,RCC1562,RCC4023,RCC449,RCC931},
	pages = {1{\textendash}10},
	abstract = {Current hypotheses suggest that cellular elemental stoichiometry of marine eukaryotic phytoplankton such as the ratios of cellular carbon:nitrogen:phosphorus (C:N:P) vary between phylogenetic groups based traits like evolutionary history and cell size. To investigate how phylogenetic structure, cell volume, growth rate and temperature interact to affect the cellular elemental stoichiometry of marine eukaryotic phytoplankton, we examined the C:N:P composition in 30 isolates across 7 classes of marine phytoplankton that were grown with a sufficient supply of nutrients with nitrate as the nitrogen source. The isolates covered a wide range in cell volume (5 orders of magnitude), growth rate ({\textexclamdown}0.01-0.9 d-1), and habitat temperature (2-24{\textdegree}C). Our analysis indicates that C:N:P is highly variable, with statistical model residuals accounting for over half of the total variance with no relationship between phylogeny and elemental stoichiometry. Furthermore, our data indicated that variability in C:P, N:P and C:N within Bacillariophyceae (diatoms) was as high as that among all of the isolates that we examined. In addition, a linear statistical model identified a positive relationship between diatom cell volume and C:P and N:P. Among all of the isolates that we examined, the statistical model identified temperature as a significant factor, consistent with the temperature-dependent translation efficiency model, but temperature only explained 5\% of the total statistical model variance. While some of our results support data from previous field studies, the high variability of elemental ratios within Bacillariophyceae contradicts previous work that suggests that this cosmopolitan group of microalgae has consistently low C:P and N:P ratios in comparison with other groups.},
	keywords = {cell size, Diatom, Dinoflagellate, eukaryote, frontiers in microbiology, frontiersin, Growth, org, protist, prymnesiophyte, RCC103, RCC1242, RCC1562, RCC4023, RCC449, RCC931, temperature, www},
	issn = {1664-302X},
	doi = {10.3389/fmicb.2018.00543},
	url = {http://journal.frontiersin.org/article/10.3389/fmicb.2018.00543/full},
	author = {Garcia, Nathan S. and Sexton, Julie and Riggins, Tracey and Brown, Jeff and Lomas, Michael W. and Martiny, Adam C.}
}
