@article {Laloui2002,
	title = {Genotyping of axenic and non-axenic isolates of the genus Prochlorococcus and the OMF-{\textquoteright}Synechococcus{\textquoteright} clade by size, sequence analysis or RFLP of the Internal Transcribed Spacer of the ribosomal operon},
	journal = {Microbiology},
	volume = {148},
	number = {2},
	year = {2002},
	note = {tex.mendeley-tags: 2002,rcc,sbr?hyto},
	pages = {453{\textendash}465},
	abstract = {PCR amplicons of the Internal Transcribed Spacer (ITS) of the rrn operon of three axenic OMF (oceanic, marine and freshwater) strains of {\textquoteright}Synechococcus{\textquoteright} (wH7803, PCC 7001 and PCC 6307, respectively) differ greatly in length from that of the axenic Prochlorococcus marinus subsp. pastoris PCC 9511(T), although these four cyanobacteria cluster relatively closely in phylogenetic trees inferred from 165 rRNA gene sequences. The ITSs of three strains (PCC 9511(T), PCC 6307 and PCC 7001) were sequenced and compared with those available for strains Prochlorococcus MED4 (CCMP 1378) and MIT9313 from genome sequencing projects. In spite of large differences in length, sequence and mean DNA base composition, conserved domains important for transcriptional antitermination and folding of the rRNA transcripts were identified in all ITSs. A new group-specific primer permitted ITS amplification even with non-axenic isolates of Prochlorococcus and one OMF-{\textquoteright}Synechococcus{\textquoteright} strain. Prochlorococcus isolates of the high-light-adapted clade (HL) differed from representatives of the low-light-adapted Glade (LL) by the length of their ITS. Restriction fragment length polymorphism (RFLP) of the ITS amplicons revealed three subclusters among the HL strains. Size, sequence data and RFLP of the ITS amplicons will therefore be valuable markers for the identification of different Prochlorococcus genotypes and for their discrimination from other cyanobacterial relatives with which they often co-exist in oceanic ecosystems.},
	keywords = {2002, Blue Green Alga, Chlorophyll B, community structure, cyanobacteria, Escherichia Coli, Marine Prokaryote, Multiple Evolutionary Origins, North Atlantic Ocean, Pacific Ocean, rcc, Rna Operon, SBR$_\textrmP$hyto, sbr?hyto},
	doi = {10.1099/00221287-148-2-453},
	author = {Laloui, W and Palinska, K A and Rippka, R and Partensky, F and de Marsac, N T and Herdman, M and Iteman, I}
}
@article {West2001,
	title = {Closely related Prochlorococcus genotypes show remarkably different depth distributions in two oceanic regions as revealed by in situ hybridization using 16S rRNA-targeted oligonucleotides},
	journal = {Microbiology - UK},
	volume = {147},
	number = {7},
	year = {2001},
	note = {tex.mendeley-tags: RCC,rcc},
	pages = {1731{\textendash}1744},
	abstract = {An in situ hybridization method was applied to the identification of marine cyanobacteria assignable to the genus Procholorococcus using harseradish-peroxidase-labelled 16S rRNA-targeted oligonucleotide probes in combination with tyramide signal amplification (TSA). With this method very bright signals were obtained, in contrast to hybridizations with oligonucleotides monolabelled with fluorochromes, which failed to give positive signals. Genotype-specific oligonucleotides for high light (HL)- and low light (LL)adapted members of this genus were identified by 16S rRNA sequence analyses and their specificities confirmed in whole-cell hybridizations with cultured strains of Prochlorococcus marinus Chisholm et al., 1992, Prochlorococcus sp. and Synechococcus sp. In situ hybridization of these genotype-specific probes to field samples from stratified water bodies collected in the North Atlantic Ocean and the Red Sea allowed a rapid assessment of the abundance and spatial distribution of HL- and LL-adapted Prochlorococcus. In both oceanic regions the LL-adapted Prochlorococcus populations were localized in deeper water whereas the HL-adapted Prochlorococcus populations were not only distinct in each region but also exhibited strikingly different depth distributions, HLI being confined to shallow wafer in the North Atlantic, in contrast to HLII, which was present throughout the water column in the Red Sea.},
	keywords = {Escherichia Coli, IDENTIFICATION, Marine Cyanobacterium, Nucleic Acid Probes, Photosynthetic Prokaryote, PICODIV, Populations, rcc, Ribosomal Rna, SEQUENCES, Tyramide Signal Amplification, Whole Cell Hybridization},
	doi = {10.1099/00221287-147-7-1731},
	author = {West, N J and Schonhuber, W A and Fuller, N J and Amann, R I and Rippka, R and Post, A F and Scanlan, D J}
}
